Zhang Z, Aoki H, Umezawa K, Kranrod J, Miyazaki N, Oshima T, Hirao T, Miura Y, Seubert J, Ito K, Aoki S. Potential role of lipophagy impairment for anticancer effects of glycolysis-suppressed pancreatic ductal adenocarcinoma cells. Cell Death Discov. 10(1):166. (2024) (Pubmed)
Kazaoka A, Fujimori S, Yamada Y, Shirayanagi T, Gao Y, Kuwahara S, Sakamoto N, Susukida T, Aoki S, Ito K. HLA-B*57:01-dependent intracellular stress in keratinocytes triggers dermal hypersensitivity reactions to Abacavir. PNAS Nexus. in press (2024) (Journal)
Kazaoka A, Kumagai K, Matsushita J, Aida T, Kuwahara S, Aoki S, Ito K. Pathological changes in various organs in HLA-B*57:01 transgenic mice with abacavir-induced skin eruption. Toxicol Res. 40(2):223-235. (2024) (Pubmed)
2023年
Takemura A, Ishii S, Ikeyama Y, Esashika K, Takahashi J, Ito K. New in vitro screening system to detect drug-induced liver injury using a culture plate with low drug sorption and high oxygen permeability. Drug Metab Pharmacokinet. 100511 (2023) (Pubmed)
Fujiwara R, Journey M, Al-Doori F, Bell P, Judge B, Miracle K, Ito K, Jones S. Potential neonatal toxicity of new psychoactive substances. Pharmacol Ther. 108468 (2023) (Pubmed)
Nakajima R, Takemura A, Ikeyama Y, Ito K. Lipopolysaccharide administration increases the susceptibility of mitochondrial permeability transition pore opening via altering adenine nucleotide translocase conformation in the mouse liver. J Toxicol Sci. 48(2):65-73 (2023) (Journal)
Nukaga T, Takemura A, Endo Y, Uesawa Y, Ito K. Estimating drug-induced liver injury risk by in vitro molecular initiation response and pharmacokinetic parameters for during early drug development. Toxicol Res. 12(1):86-94 (2023) (Journal)
2022年
Takemura A and Ito K. In Vitro Assay System to Detect Drug-Induced Bile Acid-Dependent Cytotoxicity Using Hepatocytes. In: Tanimizu, N. (eds) Hepatocytes.Methods in Molecular Biology. 2544:119-127. (2022) (Link)
Gao Y, Kuwahara S, Kazaoka A, Ito K, Aoki S. TARC/CCL17 Expression Is Associated with CD8 + T Cell Recruitment in Abacavir-Induced Skin Hypersensitivity in HLA-Transgenic Mice. Biol Pharm Bull. 45(9):1347-1353. (2022) (Pubmed)
Gao Y, Song B, Aoki S, Ito K. Conjugation of human serum albumin and flucloxacillin provokes specific immune response in HLA-B*57:01 transgenic mice. Immunol Lett. 6705. (2022) (Pubmed)
Miyazaki N, Shiratori R, Oshima T, Zhang Z, Valencia R, Kranrod J, Fang L, Seubert JM, Ito K, Aoki S. PINK1-dependent and Parkin-independent mitophagy is involved in reprogramming of glycometabolism in pancreatic cancer cells. Biochem Biophys Res Commun. 625:167-173. (2022) (Pubmed)
Shirayanagi T, Kazaoka A, Watanabe K, Qu L, Sakamoto N, Hoshino T, Ito K, Aoki S. Weak complex formation of adverse drug reaction-associated HLA-B57, B58, and B15 molecules. Toxicol In Vitro. 105383. (2022) (Pubmed)
Yamamoto C, Takemura A, Ishii S, Doi A, Saito I, Yamada H, Sakai Y, Matsunaga T, Ito K. A novel perfusion culture system for screening mitochondrial toxicity in primary mouse hepatocytes. J Toxicol Sci. 47(1):13-18. (2022) (J-STAGE)
2021年
Song B, Aoki S, Liu C, Susukida T, Kuwahara S, Ito K. The PD1 inhibitory pathway and mature dendritic cells contribute to abacavir hypersensitivity in human leukocyte antigen transgenic PD1 knockout mice. Toxicology. 152971. (2021) (Pubmed)
Susukida T, Kuwahara S, Song B, Kazaoka A, Aoki S, Ito K. Regulation of the immune tolerance system determines the susceptibility to HLA-mediated abacavir-induced skin toxicity. Commun Biol. 4(1):1137. (2021) (Pubmed)
Sato T, Takemura A, Ito K. Role of respiratory uncoupling in drug-induced mitochondrial permeability transition Toxicol Appl Pharmacol. 427, 115659. (2021) (Pubmed)
Aoki S. Importance of HLA in Determining Individual Differences in the Onset of Adverse Drug Reactions. Yakugaku Zasshi. 141(8):1001-1007. (2021) (Pubmed)
Kanamori T, Miyazaki N, Aoki S, Ito K, Hisaka A, Hatakeyama H. Investigation of energy metabolic dynamism in hyperthermia-resistant ovarian and uterine cancer cells under heat stress. Sci Rep. 11(1):14726. (2021) (Pubmed)
Takemura A, Gong S, Sato T, Kawaguchi M, Sekine S, Kazuki Y, Horie T, Ito K Evaluation of parent- and metabolite-induced mitochondrial toxicities using CYP-introduced HepG2 cells J Pharm Sci. 110(9):3306-3312. (2021) (Pubmed)
Kikuya M, Furuichi K, Hirao T, Endo S, Toyooka N, Ito K, Aoki S. Aldo-keto reductase inhibitors increase the anticancer effects of tyrosine kinase inhibitors in chronic myelogenous leukemia J Pharmacol Sci. 147(1):1-8. (2021) (Pubmed)
Deguchi S, Shintani T, Harada K, Okamoto T, Takemura A, Hirata K, Ito K, Takayama K, Mizuguchi H. In Vitro Model for a Drug Assessment of Cytochrome P450 Family 3 Subfamily A Member 4 Substrates Using Human Induced Pluripotent Stem Cells and Genome Editing Technology Hepatol Commun. 5(8):1385-1399. (2021) (Journal)
2020年
Susukida T, Aoki S, Shirayanagi T, Yamada Y, Kuwahara S, Ito K. HLA transgenic mice: application in reproducing idiosyncratic drug toxicity Drug Metab Rev. 52(4):540-567. (2020) (PubMed)
Shirayanagi T, Aoki S, Fujimori S, Watanabe K, Aida T, Hirasawa M, Kumagai K, Hoshino T, Ito K. Detection of Abacavir-Induced Structural Alterations in Human Leukocyte Antigen-B*57:01 Using Phage Display Biol Pharm Bull. 43(6):1007-1015. (2020) (PubMed)
Ikeyama Y, Sato T, Takemura A, Sekine S, Ito K. Hypoxia/reoxygenation exacerbates drug-induced cytotoxicity by opening mitochondrial permeability transition pore: Possible application for toxicity screening Toxicol In Vitro. 67, 104889 (2020) (PubMed)
Aoki S, Shimizu K, Ito K. Autophagy-dependent Mitochondrial Function Regulates Osteoclast Differentiation and Maturation Biochem Biophys Res Commun. 527(4):874-880. (2020) (PubMed)
Kawaguchi M, Nukaga T, Sekine S, Takemura A, Susukida T, Oeda S, Kodama A, Hirota M, Kouzuki H, Ito K. Mechanism-based integrated assay systems for the prediction of drug-induced liver injury. Toxicol Appl Pharmacol. 114958 (2020) (PubMed)
Gao Y, Song B, Aoki S, Ito K. Role of Kupffer cells in liver injury induced by CpG oligodeoxynucleotide and flucloxacillin in mice. EXCLI J. 19, 387-399. (2020) (Journal)
Ikeyama Y, Sato T, Takemura A, Sekine S, Ito K. Successful energy shift from glycolysis to mitochondrial oxidative phosphorylation in freshly isolated hepatocytes from humanized mice liver. Toxicol In Vitro. 65, 104785 (2020) (PubMed)
2019年
Shiratori R, Furuichi K, Yamaguchi M, Miyazaki N, Aoki H, Chibana H, Ito K, Aoki S. Glycolytic Suppression Dramatically Changes the Intracellular Metabolic Profile of Multiple Cancer Cell Lines in a Mitochondrial Metabolism-Dependent Manner Sci Rep. 9(1), 18699 (2019)(PubMed)
Arakawa K, Ikeyama Y, Sato T, Segawa M, Sekine S, Ito K. Functional modulation of liver mitochondria in lipopolysaccharide/drug co-treated rat liver injury model J Toxicol Sci. 44(12):833-843 (2019)(PubMed)
Sato T, Segawa M, Sekine S, Ito K. Mild Depolarization Is Involved in Troglitazone-Induced Liver Mitochondrial Membrane Permeability Transition via Mitochondrial iPLA2 Activation J Toxicol Sci. 44(11):811-820 (2019)(PubMed)
Sato T, Takemura A, Ikeyama Y, Sakamaki Y, Mimata A, Aoyagi H, Aizaki H, Sekine S, Ito K. Transmission electron microscopy of the benzbromarone-induced change in mitochondrial morphology in HepG2 cells. Fundam. Toxicol. Sci. 6(8):281-286. (2019)(J-STAGE)
Sakai Y, Iwao T, Susukida T, Nukaga T, Takemura A, Sekine S, Ito K, Matsunaga T. In vitro bile acid-dependent hepatocyte toxicity assay system using human induced pluripotent stem cell-derived hepatocytes: Current status and disadvantages to overcome. Drug Metab Pharmacokinet. 34(4):264-271. (2019)(PubMed)
Sakai Y, Okumura H, Iwao T, Watashi K, Ito K, Matsunaga T. Development of an in vitro cholestatic drug-induced liver injury evaluation system using HepG2-hNTCP-C4 cells in sandwich configuration. Toxicol In Vitro. 61:104619. (2019)(PubMed)
Takemura A, Gong S, Sekine S, Ito K. Inhibition of biliary network reconstruction by benzbromarone delays recovery from pre-existing liver injury. Toxicology. 423:32-41. (2019)(PubMed)
Song B, Aoki S, Liu C, Ito K. A toll-like receptor 9 agonist sensitizes mice to mitochondrial dysfunction-induced hepatic apoptosis via the Fas/FasL pathway. Arch Toxicol. 93(6):1573-1584. (2019)(PubMed)
2018年
Ohe T, Umezawa R, Kitagawara Y, Yasuda D, Takahashi K, Nakamura S, Abe A, Sekine S, Ito K, Okunushi K, Morio H, Furihata T, Anzai N, Mashino T. Synthesis of novel benzbromarone derivatives designed to avoid metabolic activation. Bioorg Med Chem Lett. 28(23-24):3708-3711. (2018)(PubMed)
Aoto K, Ito K, Aoki S. Complex formation between platelet-derived growth factor receptor β and transforming growth factor β receptor regulates the differentiation of mesenchymal stem cells into cancer-associated fibroblasts. Oncotarget. 9(75):34090-34102. (2018)(Journal)
Ikebuchi Y, Aoki S, Honma M, Hayashi M, Sugamori Y, Khan M, Kariya Y, Kato G, Tabata Y, Penninger JM, Udagawa N, Aoki K, Suzuki H. Coupling of bone resorption and formation by RANKL reverse signalling. Nature. 561(7722):195-200. (2018)(Pubmed)
Song B, Aoki S, Liu C, Susukida T, Ito K. An animal model of abacavir-induced HLA-mediated liver injury. Toxicol Sci. 162(2):713-723. (2018)(PubMed)
Segawa M, Sekine S, Sato T, Ito K. Increased susceptibility to troglitazone-induced mitochondrial permeability transition in type 2 diabetes mellitus model rat. J Toxicol Sci. 43(5):339-351. (2018)(Pubmed)
2017年
Aoki S, Morita M, Hirao T, Yamaguchi M, Shiratori R, Kikuya M, Chibana H, Ito K. Shift in energy metabolism caused by glucocorticoids enhances the effect of cytotoxic anti-cancer drugs against acute lymphoblastic leukemia cells. Oncotarget. 8(55):94271-94285. (2017)(PubMed)
Hirao T, Yamaguchi M, Kikuya M, Chibana H, Ito K, Aoki S. Altered intracellular signaling by imatinib increases the anti-cancer effects of tyrosine kinase inhibitors in CML cells. Cancer Sci. 109(1):121-131. (2017)(PubMed)
Susukida T, Aoki S, Kogo K, Fujimori S, Song B, Liu C, Sekine S, Ito K. Evaluation of immune-mediated idiosyncratic drug toxicity using chimeric HLA transgenic mice. Arch Toxicol. 92(3):1177-1188. (2017)(PubMed)
Liu C, Sekine S, Song B, Ito K. Use of Primary Rat Hepatocytes for Prediction of Drug-Induced Mitochondrial Dysfunction. Curr Protoc Toxicol. 72(1):14.16.1-14.16.10. (2017)(PubMed)
Ogimura E, Tokizono M, Sekine S, Nakagawa T, Bando K, Ito K. Metabolic Activation of Cholestatic Drug-induced Bile Acid-dependent Toxicity in Human Sandwich-Cultured Hepatocytes. J Pharm Sci. 106(9):2509-2514. (2017)(Pubmed)
Ogimura E, Nakagawa T, Deguchi J, Sekine S, Ito K, Bando K. Troglitazone inhibits bile acid amidation: a possible risk factor for liver injury. Toxicol Sci. 158(2):347-355. (2017)(Pubmed)
Oizumi K, Sekine S, Fukagai M, Susukida T, Ito K. Identification of bile acids responsible for inhibiting the bile salt export pump, leading to bile acid accumulation and cell toxicity in rat hepatocytes. J Pharm Sci. 106(9):2412-2419. (2017)(Pubmed)
2016年
Susukida T, Sekine S, Nozaki M, Tokizono M, Oizumi K, Horie T, Ito K. Establishment of a Drug-Induced, Bile Acid-Dependent Hepatotoxicity Model Using HepaRG Cells. J Pharm Sci. 105(4):1550-1560, (2016).(PubMed)
Liu C, Sekine S, Ito K. Assessment of mitochondrial dysfunction-related, drug-induced hepatotoxicity in primary rat hepatocytes. Toxicol Appl Pharmacol. 302:23-30, (2016)(PubMed)
Kawaguchi M, Aoki S, Hirao T, Morita M, Ito K. Autophagy is an important metabolic pathway to determine leukemia cell survival following suppression of the glycolytic pathway. Biochem Biophys Res Commun. 474 (1) 188-192 (2016).(PubMed)
Takemura A, Izaki A, Sekine S, Ito K. Inhibition of bile canalicular network formation in rat sandwich cultured hepatocytes by drugs associated with risk of severe liver injury. Toxicol In Vitro 35, 121-30 (2016)(PubMed)
Sugamori Y, Mise-Omata S, Maeda C, Aoki S, Tabata Y, Murali R, Yasuda H, Udagawa N, Suzuki H, Honma M, Aoki K. Peptide drugs accelerate BMP-2-induced calvarial bone regeneration and stimulate osteoblast differentiation through mTORC1 signaling. Bioessays.38 (8), 717-25 (2016) (PubMed)
Takamatsu D, Kobayashi E, Ito K, Satoh N. The survey on actual status of use and patient' attitude towards electronic medicine notebook. RSMP 6: 139-150, (2016)(J-STAGE)
2015年
Sekine S, Ito K, Watanabe H, Nakano T, Moriya K, Shintani Y, Fujie H, Tsutsumi T, Miyoshi H, Fujinaga H, Shinzawa S, Koike K, Horie T. Mitochondrial iron accumulation exacerbates hepatic toxicity caused by hepatitis C virus core protein. Toxicol Appl Pharmacol. 282(3):237-43, (2015). (PubMed)
Kosaka K, Watanabe T, Susukida T, Aoki S, Sekine S, Kume T, Ito K. Key determinants of the circulatory exposure of organic anions: differences in hepatic uptake between multidrug resistance-associated protein 2 (Mrp2)-deficient rats and wild-type rats. Xenobiotica. 45 (6), 556-62 (2015). (PubMed)
Niu X, de Graaf IA, van de Vegte D, Langelaar-Makkinje M, Sekine S, Groothuis GM. Consequences of Mrp2 deficiency for diclofenac toxicity in the rat intestine ex vivo. Toxicol In Vitro. 29(1):168-175, (2015). (PubMed)
Ito N, Ito K, Ikebuchi Y, Toyoda Y, Takada T, Hisaka A, Oka A, Suzuki H. Prediction of Drug Transfer into Milk Considering Breast Cancer Resistance Protein (BCRP)-Mediated Transport. Pharm Res. 32 (8), 2527-37 (2015). (PubMed)
Kato K, Moriyama C, Ito N, Zhang X, Hachiuma K, Hagima N, Iwata K, Yamaguchi JI, Maeda K, Ito K, Suzuki H, Sugiyama Y, Kusuhara H. Involvement of Organic Cation Transporters in the Clearance and Milk Secretion of Thiamine in Mice. Pharm Res. 32 (7), 2192-204 (2015). (PubMed)
Susukida T, Sekine S, Ogimura E, Aoki S, Oizumi K, Horie T, Ito K. Basal efflux of bile acids contributes to drug-induced bile acid-dependent hepatocyte toxicity in rat sandwich-cultured hepatocytes. Toxicol In Vitro. 29(7):1454-63, (2015). (PubMed)
Shirakawa M, Sekine S, Tanaka A, Horie T, Ito K. Metabolic activation of hepatotoxic drug (benzbromarone) induced mitochondrial membrane permeability transition.Toxicol Appl Pharmacol. 288(1):12-18, (2015). (PubMed)
Oga EF, Sekine S, Shitara Y, Horie T.Pharmacokinetic Herb-Drug Interactions: Insight into Mechanisms and Consequences.Eur J Drug Metab Pharmacokinet. 41(2), 93-108 (2015). (PubMed)
Susukida T, Sekine S, Nozaki M, Tokizono M, Ito K. Prediction of the Clinical Risk of Drug-Induced Cholestatic Liver Injury Using an In Vitro Sandwich Cultured Hepatocyte Assay.Drug Metab Dispos. 43(11):1760-8, (2015). (PubMed)
Belal US, Norose K, Mohamed RM, Sekine S, Nukaga T, Ito K, Abdellatif MZ, Abdelgelil NH, Yano A.Quantitative assessment of the effects of sulfamethoxazole on Toxoplasma gondii loads in susceptible WT C57BL/6 mice as an immunocompetent host model.Parasitol Int. 65 (1), 1-4 (2015). (PubMed)
2014年以前
Ito N, Ito K, Ikebuchi Y, Kito T, Miyata H, Toyoda Y, Takada T, Hisaka A, Honma M, Oka A, Kusuhara H, Suzuki H. Organic cation transporter/solute carrier family 22a is involved in drug transfer into milk in mice. J Pharm Sci. 103(10):3342-8, (2014). (PubMed)
Oga EF, Sekine S, Horie T. Ex vivo and in vivo investigations of the effects of extracts of Vernonia amygdalina, Carica papaya and Tapinanthus sessilifolius on digoxin transport and pharmacokinetics: assessing the significance on rat intestinal P-glycoprotein efflux. Drug Metab Pharmacokinet. 28(4):314-20, (2013). (PubMed)
Hamada K, Kakigawa N, Sekine S, Shitara Y, Horie T. Disruption of ZO-1/claudin-4 interaction in relation to inflammatory responses in methotrexate-induced intestinal mucositis. Cancer Chemother Pharmacol. 72(4):757-65, (2013). (PubMed)
Sekine S, Kimura T, Motoyama M, Shitara Y, Wakazono H, Oida H, Horie T. The role of cyclophilin D in interspecies differences in susceptibility to hepatotoxic drug-induced mitochondrial injury. Biochem Pharmacol. 86(10):1507-14, (2013). (PubMed)
Nakano T, Sekine S, Ito K, Horie T. Ezrin regulates the expression of Mrp2/Abcc2 and Mdr1/Abcb1 along the rat small intestinal tract. Am J Physiol Gastrointest Liver Physiol. 305(11):G807-17, (2013). (PubMed)
Oga EF, Sekine S, Shitara Y, Horie T. Potential P-glycoprotein-mediated drug-drug interactions of antimalarial agents in Caco-2 cells. Am J Trop Med Hyg. 87(1):64-9, (2012). (PubMed)
Ueda K, Higashi K, Limwikrant W, Sekine S, Horie T, Yamamoto K, Moribe K. Mechanistic differences in permeation behavior of supersaturated and solubilized solutions of carbamazepine revealed by nuclear magnetic resonance measurements. Mol Pharm. 9(11):3023-33, (2012). (PubMed)
Oga EF, Sekine S, Shitara Y, Horie T. P-glycoprotein mediated efflux in Caco-2 cell monolayers: the influence of herbals on digoxin transport. J Ethnopharmacol. 144(3):612-7, (2012). (PubMed)
Sekine S, Mitsuki K, Ito K, Kugioka S, Horie T. Sustained intrahepatic glutathione depletion causes proteasomal degradation of multidrug resistance-associated protein 2 in rat liver. Biochim Biophys Acta. 1822(6):980-7, (2012). (PubMed)
Ogimura E, Sekine S, Horie T. Bile salt export pump inhibitors are associated with bile acid-dependent drug-induced toxicity in sandwich-cultured hepatocytes. Biochem Biophys Res Commun. 416(3-4):313-7, (2011). (PubMed)
Sekine S, Ito K, Saeki J, Horie T. Interaction of Mrp2 with radixin causes reversible canalicular Mrp2 localization induced by intracellular redox status. Biochim Biophys Acta. 1812(11):1427-34, (2011). (PubMed)
Saeki J, Sekine S, Horie T. LPS-induced dissociation of multidrug resistance-associated protein 2 (Mrp2) and radixin is associated with Mrp2 selective internalization in rats. Biochem Pharmacol. 81(1):178-84, (2011). (PubMed)
Maeda T, Miyazono Y, Ito K, Hamada K, Sekine S, Horie T. Oxidative stress and enhanced paracellular permeability in the small intestine of methotrexate-treated rats. Cancer Chemother Pharmacol. 65(6):1117-23, (2010). (PubMed)
Yano K, Sekine S, Nemoto K, Fuwa T, Horie T. The effect of dimerumic acid on LPS-induced downregulation of Mrp2 in the rat. Biochem Pharmacol. 80(4):533-9, (2010). (PubMed)
Sekine S, Yano K, Saeki J, Hashimoto N, Fuwa T, Horie T. Oxidative stress is a triggering factor for LPS-induced Mrp2 internalization in the cryopreserved rat and human liver slices. Biochem Biophys Res Commun. 399(2):279-85, (2010). (PubMed)
Hamada K, Shitara Y, Sekine S, Horie T. Zonula Occludens-1 alterations and enhanced intestinal permeability in methotrexate-treated rats. Cancer Chemother Pharmacol. 66(6):1031-8, (2010). (PubMed)
Nakano T, Sekine S, Ito K, Horie T. Correlation between apical localization of Abcc2/Mrp2 and phosphorylation status of ezrin in rat intestine. Drug Metab Dispos. 37(7):1521-7, (2009). (PubMed)
Sekine S, Ito K, Horie T. Canalicular Mrp2 localization is reversibly regulated by the intracellular redox status. Am J Physiol Gastrointest Liver Physiol. 295(5):1035-41, (2008). (PubMed)
Sekine S, Ito K and Horie T. Oxidative stress and Mrp2 internalization. Free Radic Biol Med. 40(12):2166-74, (2006). (PubMed)
Ito K, Suzuki H, Horie T, Sugiyama Y. Apical/basolateral surface expression of drug transporters and its role in vectorial drug transport. Pharm Res. 22(10):1559-77, (2005). (PubMed)
Ji B, Ito K, Sekine S, Tajima A, Horie T. Ethacrynic-acid-induced glutathione depletion and oxidative stress in normal and Mrp2-deficient rat liver. Free Radic Biol Med. 37(11):1718-29, (2004). (PubMed)
