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Hiroto Kawashima

 
Laboratory of Microbiology and Immunology
Graduate School of Pharmaceutical Sciences, Chiba University 　　　

March 1988
　Graduated from Department of Pharmacy, University of Tokyo
April 1988
　Graduate School of Pharmaceutical Sciences, The University of Tokyo Admission to the Department of Life Pharmacy
March　1990
　Completed the master course
April 1990 
　University of Tokyo Graduate School of Pharmaceutical Sciences  Ph.D.
March 1993
　Completed the doctoral course and acquire a doctorate (pharmacy) degree
April 1993 - July 1994
　Tokyo Independent Medical Research Institute Medical Research Institute Immunological Research Division Researcher
August 1994 - September 2001
　Osaka University Graduate School of Medicine Graduate School of Organ Control Department Assistant
October 2001 - March 2005
　The Burnham Institute Researcher (Postdoctoral Associate)
April 2005 - April 2014
　Associate Professor (Department of Immunology and Microbiology, Shizuoka Prefectural University School of Pharmacy) Associate Professor (Institute of Pharmaceutical Sciences, Shizuoka Prefectural University)
October 2006 - March 2010
　JST PRESTO researcher (concurrently)
May 2014 - July 2015
　Professor of Hoshi University School of Pharmaceutical Sciences Professor of Biochemistry
August 2015 - Currently
　Professor of Immunological Microbiology Laboratory, Graduate School of Pharmaceutical Sciences, Chiba University

A series of studies focusing on the function of sugar chain in lymphocyte homing revealed that chondroitin sulfate proteoglycan is an important regulator of lymphocyte homing (J. Biol. Chem. 2000, 2002), as well as glycoprotein Sulfation at position 6 of GlcNAc of sugar chain proved to have essential function in lymphocyte homing (Nat. Immunol. 2005). We succeeded in establishing various anti-sugar chain antibodies based on a new methodology and clarified the function of specific sugar chain in lymphocyte homing in more detail (J. Biol. Chem. 2010). Recently, we succeeded in producing high endothelial vena (HEV) -specific heparan sulfate-deficient mice and proved that heparan sulfate functions to promote lymphocyte homing by presenting chemokines in the HEV lumen (J. Immunol . 2013).

【Paper】
Tsuboi K, Nishitani M, Takakura A, Imai Y, Komatsu M and Kawashima H. Autophagy protects against colitis by the maintenance of normal gut microflora and secretion of mucus.
J. Biol. Chem., 290:20511-20526, 2015.

Matsumura R, Hirakawa J, Sato K, Ikeda T, Nagai M, Fukuda M, Imai Y and Kawashima H. Novel antibodies reactive with sialyl Lewis X in both humans and mice define its critical role in leukocyte trafficking and contact hypersensitivity responses.
J. Biol. Chem., 290:15313-15326, 2015.

Tsuboi K., Hirakawa J., Seki E., Imai Y., Yamaguchi Y., Fukuda M., and Kawashima H. Role of high　endothelial venule-expressed heparan sulfate in chemokine presentation and lymphocyte homing.
J. Immunol., 191:448-455, 2013.

Ohmichi Y., Hirakawa J., Imai Y., Fukuda M., and Kawashima H. Essential role of peripheral node addressin in lymphocyte homing to nasal-associated lymphoid tissues and allergic immune responses.
J. Exp. Med., 208:1015-1025, 2011.

Hirakawa J., Tsuboi K., Sato K., Kobayashi M., Watanabe S., Takakura A., Imai Y., Ito Y., Fukuda M., and Kawashima H. Novel anti-carbohydrate antibodies reveal the cooperative function of sulfated N- and O-glycans in lymphocyte homing.
J. Biol. Chem., 285:40864-40878, 2010.

Tobisawa Y., Imai Y., Fukuda M., and Kawashima H. Sulfation of colonic mucins by N-acetylglucosamine-6-O-sulfotransferase-2 and its protective function in experimental colitis in mice.
J. Biol. Chem., 285:6750-6760, 2010.

Kawashima H., Hirakawa J., Tobisawa Y., Fukuda M., and Saga Y.. Conditional gene targeting in mouse high endothelial venules.
J. Immunol., 182:5461-5468, 2009.

Mitoma J., Bao X., Petryanik B., Schaerli P., Gauguet J.M., Yu S.Y., Kawashima H., Saito H., Ohtsubo K., Marth J.D., Khoo K.H., von Andrian U.H., Lowe J.B., and Fukuda M. Critical functions of N-glycans in L-selectin-mediated lymphocyte homing and recruitment.
Nature Immunology, 8:409-418, 2007.

Kawashima H., Petryniak B., Hiraoka N., Mitoma J., Huckaby V., Nakayama J., Uchimura K., Kadomatsu K., Muramatsu T., Lowe J.B., and Fukuda M. N-Acetylglucosamine-6-O-sulfotransferases 1 and 2 cooperatively control lymphocyte homing through L-selectin ligand biosynthesis in high endothelial venules.
Nature Immunology 6:1096-1104, 2005.

Kawashima H., Watanabe N., Hirose M., Sun X., Atarashi K., Kimura T., Shikata K., Matsuda M., Ogawa D., Heljasvaara R., Rehn M., Pihlajaniemi T., and Miyasaka M. Collagen XVIII, a basement membrane heparan sulfate proteoglycan, interacts with L-selectin and monocyte chemoattractant protein-1.
J. Biol. Chem. 278:13069-13076, 2003.

Kawashima H., Atarashi K., Hirose M., Hirose J., Yamada S., Sugahara K., and Miyasaka M. Oversulfated chondroitin/dermatan sulfates containing GlcAβ1/IdoAα1-3GalNAc(4,6-O-disulfate) interact with L- and P-selectin and chemokines.
J. Biol. Chem. 277:12921-12930, 2002.

Kawashima H., Hirose M., Hirose J., Nagakubo D., Plaas H.K.A., and Miyasaka M. Binding of a large chondroitin sulfate/dermatan sulfate proteoglycan, versican, to L-selectin, P-selectin, and CD44.
J. Biol. Chem. 275:35448-35456, 2000.

【Book】
Kawashima H. Analysis of L-selectin-mediated cellular interactions under flow conditions.
Methods in Molecular Biology, Lectins (Hirabayashi J, ed.), Humana Press. 1200:401-412, 2014.

Kawashima H. Generation of anti-sulfated glycan antibodies using sulfotransferase-deficient mice.
Methods in Molecular Biology, 1022:51-60, 2013.

Kawashima H. Roles of GlcNAc-6-O-sulfotransferases in lymphoid and nonlymphoid tissues.
Methods in Enzymology 479:243-256, 2010.

Kawashima H. Determination of chemokine-glycosaminoglycan interaction specificity.
Methods in Enzymology 416:254-263, 2006.

Kawashima H. Functions of glycans revealed by gene inactivation of L-selectin ligand sulfotransferases in mice.
Methods in Enzymology 416:279-290, 2006.